Changeset 238
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INSTALL
r227 r238 4 4 5 5 Note: When using Mac OS-X and installing MAKER you must either be a system 6 administrator with sudo privleges or be logged in as root on the terminal for some7 parts of the installation.6 administrator with sudo privleges or be logged in as root on the terminal for 7 some parts of the installation. 8 8 9 9 … … 20 20 a. Download and unpack the most recent BioPerl package to a directory of your 21 21 choice, or use Subversion to get the most current version of BioPerl. See 22 http://www.bioperl.org for details on how to download using Subversion. You23 will then need to set PERL5LIB in your .bash_profile to the location of24 bioperl (i.e. export PERL5LIB="/usr/local/bioperl-live:$PERL5LIB").22 http://www.bioperl.org for details on how to download using Subversion. 23 You will then need to set PERL5LIB in your .bash_profile to the location 24 of bioperl (i.e. export PERL5LIB="/usr/local/bioperl-live:$PERL5LIB"). 25 25 26 26 -full BioPerl instalation- … … 34 34 b. Type install Bundle::CPAN on the cpan command line. Once again just press 35 35 enter to accept default installation options. 36 c. Type install Module::Build on the cpan command line. Once again just press37 enter to accept default installation options.36 c. Type install Module::Build on the cpan command line. Once again just 37 press enter to accept default installation options. 38 38 d. Type install Bundle::BioPerl on the cpan command line. Once again press 39 39 enter to accept default installation options. 40 40 e. Unpack the downloaded bioperl tar file to the directory of your choice or 41 41 use Subversion to get the most up to date version. Then use the terminal 42 to find the directory and type perl Build.PL in the command line, then type43 ./Build test, then if all tests pass, type ./Build install. This will44 install BioPerl where perl is installed. Press enter to accept all42 to find the directory and type perl Build.PL in the command line, then 43 type ./Build test, then if all tests pass, type ./Build install. This 44 will install BioPerl where perl is installed. Press enter to accept all 45 45 defaults. For Mac OS-X BioPerl will install to /Library/Perl/5.8.6/. 46 46 47 47 48 48 3. Install WuBlast 2.0 or higher 49 (WuBlast has become AB-Blast and is no longer freely available, so if you are50 not lucky enough to have an existing copy of WuBlast, you can use NCBI BLAST51 instead. See NCBI BLAST documentation for installation instructions. It is52 important to note that WuBlast is a prerequisite for RepeatMasker53 installation, so if you do not have WuBlast, then you will have to install54 Cross_match to get RepeatMasker to work. See RepeatMasker documentation.)49 (WuBlast has become AB-Blast and is no longer freely available, so if you 50 are not lucky enough to have an existing copy of WuBlast, you can use NCBI 51 BLAST instead. See NCBI BLAST documentation for installation instructions. 52 It is important to note that WuBlast is a prerequisite for RepeatMasker 53 installation, so if you do not have WuBlast, then you will have to install 54 Cross_match to get RepeatMasker to work. See RepeatMasker documentation.) 55 55 56 56 a. Unpack the tar file into the directory of your choice (i.e. /usr/local). 57 b. Add the following in your .bash_profile (value depend on where you chose to58 install wublast):57 b. Add the following in your .bash_profile (value depend on where you chose 58 to install wublast): 59 59 export WUBLASTFILTER="/usr/local/wublast/filter" 60 60 export WUBLASTMAT="/usr/local/wublast/matrix" … … 65 65 4. Install SNAP. Download from http://homepage.mac.com/iankorf/ 66 66 67 a. Unpack the SNAP tar file into the directory of your choice (i .e./usr/local)67 a. Unpack the SNAP tar file into the directory of your choice (ie /usr/local) 68 68 b. Add the following to your .bash_profile file (value depends on where you 69 69 choose to install snap): export ZOE="/usr/local/snap/Zoe" 70 c. Navigate to the directory where snap was unpacked (i.e. /usr/local/snap) and71 type make70 c. Navigate to the directory where snap was unpacked (i.e. /usr/local/snap) 71 and type make 72 72 d. Add the location where you installed SNAP to your PATH variable in 73 73 .bash_profile (i.e. export PATH="/usr/local/snap:$PATH"). … … 76 76 5. Install RepeatMasker. Download from http://www.repeatmasker.org 77 77 78 a. The most current version of RepeatMasker requires a program called TRF. This79 can be downloaded from http://tandem.bu.edu/trf/trf.html78 a. The most current version of RepeatMasker requires a program called TRF. 79 This can be downloaded from http://tandem.bu.edu/trf/trf.html 80 80 b. The TRF download will contain a single executable file. You will need to 81 81 rename the file from whatever it is to just 'trf' (all lower case). … … 83 83 wherever you want. I just put it in the /RepeatMasker directory. 84 84 d. Unpack RepeatMasker to the directory of your choice (i.e. /usr/local). 85 e. Now in the RepeatMasker directory type perl ./configure in the command line.86 You will be asked to identify the location of perl, wublast, and trf. The87 script expects the paths to the folders containing the executables (because88 you are pointing to a folder the path must end in a '/' character or the89 configuration script throws a fit).85 e. Now in the RepeatMasker directory type perl ./configure in the command 86 line. You will be asked to identify the location of perl, wublast, and 87 trf. The script expects the paths to the folders containing the 88 executables (because you are pointing to a folder the path must end in a 89 '/' character or the configuration script throws a fit). 90 90 f. Add the location where you installed RepeatMasker to your PATH variable in 91 91 .bash_profile (i.e. export PATH="/usr/local/RepeatMasker:$PATH"). 92 g. You must register at http://www.girinst.org and download the Repbase repeat93 database, Repeat Masker edition, for RepeatMasker to work.94 h. Unpack the contents of the RepBase tarball into the RepeatMasker/Libraries /92 g. You must register at http://www.girinst.org and download the Repbase 93 repeat database, Repeat Masker edition, for RepeatMasker to work. 94 h. Unpack the contents of the RepBase tarball into the RepeatMasker/Libraries 95 95 directory. 96 96 … … 99 99 http://www.ebi.ac.uk/~guy/exonerate 100 100 101 a. Exonerate has pre-comiled binaries for many systems; however; for Mac OS-X ,101 a. Exonerate has pre-comiled binaries for many systems; however; for Mac OS-X 102 102 you will have to download the source code and complile it yourself by 103 103 following steps b though d. 104 b. You need to have Glib and Glib 2.0 installed. The easiest way to do this is 105 to install fink and then type fink install glib and fink install glib2-dev. 104 b. You need to have Glib and Glib 2.0 installed. The easiest way to do this 105 is to install fink and then type fink install glib and fink install 106 glib2-dev. 106 107 c. Change to the directory containing the Exonerate package to be compiled. 107 108 d. To install exonerate in the directory /usr/local/exonerate, type: 108 ./configure -prefix=/usr/local/exonerate -> then type make -> then type make109 install109 ./configure -prefix=/usr/local/exonerate -> then type make -> then type 110 make install 110 111 e. Add the location where you installed exonerate to your PATH variable in 111 112 .bash_profile (i.e. export PATH="/usr/local/exonerate/bin:$PATH"). … … 124 125 export AUGUSTUS_CONFIG_PATH="where_augustus_is/config 125 126 d. Add the location where you installed MAKER to your PATH variable in 126 .bash_profile (i.e. export PATH= "/usr/local/maker/bin:$PATH").127 .bash_profile (i.e. export PATH=/usr/local/maker/bin:$PATH). 127 128 e. You can now run a test of MAKER by following the instructions in the MAKER 128 129 README file. … … 139 140 b. Unpack Augustus to the directory of your choice (i.e. /usr/local). 140 141 c. Change to the src/ directory inside the extracted augustus folder. 141 d. Install and compile Augustus by typing: make -> then type chmod +x augustus 142 etraining -> then type cp augustus etraining ../bin 142 d. Install and compile Augustus by typing: make 143 143 e. Add the following to your .bash_profile file (value depends on where you 144 install augutus): export AUGUSTUS_CONFIG_PATH= "/usr/local/augustus/config"144 install augutus): export AUGUSTUS_CONFIG_PATH=/usr/local/augustus/config 145 145 f. Add the location where you installed augustus to your PATH variable in 146 .bash_profile (i.e. export PATH= "/usr/local/augustus/bin:$PATH").146 .bash_profile (i.e. export PATH=/usr/local/augustus/bin:$PATH). 147 147 148 148
