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use strict; |
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use warnings; |
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use Getopt::Long; |
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my $usage = " |
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; |
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my ($help, $prefix, $justify, $sort_order); |
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my $opt_success = GetOptions('help' => \$help, |
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'prefix=s' => \$prefix, |
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'justify=s' => \$justify, |
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'sort_order=s' => \$sort_order, |
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); |
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die $usage if $help || ! $opt_success || ! $prefix; |
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$justify ||= 6; |
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my $gff_file = shift; |
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die $usage unless $gff_file; |
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my $sort_map = parse_sort_order($sort_order, $gff_file); |
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open (my $IN, '<', $gff_file) or die "Can't open $gff_file for reading\n$!\n"; |
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my %counter; |
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my %ids; |
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while (<$IN>) { |
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last if /^\ |
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next if /^\s*\ |
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my ($seq, $source, $type, $start, $end, $score, $strand, |
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$phase, $attrb_text) = split /\t/, $_; |
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if ($type =~ /^(gene|mRNA)$/) { |
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my ($id) = $attrb_text =~ /ID=(.*?);/; |
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$ids{$seq}{$start}{$id} = $type; |
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} |
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} |
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for my $contig_id (sort {sort_contigs($a, $b, $sort_map)} keys %ids) { |
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my $contig = $ids{$contig_id}; |
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for my $start (sort {$b <=> $a} keys %{$contig}) { |
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for my $id (keys %{$contig->{$start}}) { |
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my $type = $contig->{$start}{$id}; |
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my $abrv = $type eq 'gene' ? 'G' : 'T'; |
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my $count = sprintf '%06s', ++$counter{$type}; |
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my $new_id = 'PYU1_' . $abrv . $count; |
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print "$id\t$new_id\n"; |
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print ''; |
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} |
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} |
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} |
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sub parse_sort_order { |
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my ($sort_order_file, $gff_file) = @_; |
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my %sort_order; |
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if ($sort_order_file) { |
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open(my $IN, '<', $sort_order_file) or die "Can't open $sort_order_file\n$!\n"; |
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my %sort_order = (<$IN>); |
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close $IN; |
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} |
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elsif (`grep -P '\#\#sequence-region' $gff_file`) { |
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open(my $IN, '<', $gff_file) or die "Can't open $gff_file\n$!\n"; |
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my $flag; |
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while (my $line = <$IN>) { |
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if ($line =~ /\ |
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$flag++; |
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my ($contig_id, $start, $end) = ($1, $2, $3); |
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$sort_order{$contig_id} = ($end - $start); |
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} |
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last if $flag; |
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} |
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close $IN; |
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} |
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else { |
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open(my $IN, '<', $gff_file) or die "Can't open $gff_file\n$!\n"; |
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while (<$IN>) { |
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my @fields = split /\t/, $_; |
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next unless @fields == 9; |
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my $seq = $fields[0]; |
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$sort_order{$seq}++; |
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} |
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} |
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return \%sort_order; |
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} |
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sub sort_contigs { |
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my ($a, $b, $sort_map) = @_; |
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return ($sort_order->{$a} <=> $sort_map->{$b}) if defined $sort_order; |
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return ($sort_order->{$b} <=> $sort_map->{$a}); |
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} |
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